pCDNA3.1-H-Luc_cyto
(Plasmid
#113929)
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PurposePlasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerThermoFisher Scientific
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6933
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Luc
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SpeciesSynthetic
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Insert Size (bp)1437
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-H-Luc_cyto was a gift from Kai Johnsson (Addgene plasmid # 113929 ; http://n2t.net/addgene:113929 ; RRID:Addgene_113929) -
For your References section:
Luciferases with Tunable Emission Wavelengths. Hiblot J, Yu Q, Sabbadini MDB, Reymond L, Xue L, Schena A, Sallin O, Hill N, Griss R, Johnsson K. Angew Chem Int Ed Engl. 2017 Nov 13;56(46):14556-14560. doi: 10.1002/anie.201708277. Epub 2017 Oct 9. 10.1002/anie.201708277 PubMed 28941028
