pET51b(+)_MBP-nLucCP-65/68
(Plasmid
#113921)
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PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113921 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET51-b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5218
- Total vector size (bp) 6778
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMBP-NanoLucCP-65/68
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SpeciesSynthetic
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Insert Size (bp)1716
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer pET-RP (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET51b(+)_MBP-nLucCP-65/68 was a gift from Kai Johnsson (Addgene plasmid # 113921 ; http://n2t.net/addgene:113921 ; RRID:Addgene_113921) -
For your References section:
Luciferases with Tunable Emission Wavelengths. Hiblot J, Yu Q, Sabbadini MDB, Reymond L, Xue L, Schena A, Sallin O, Hill N, Griss R, Johnsson K. Angew Chem Int Ed Engl. 2017 Nov 13;56(46):14556-14560. doi: 10.1002/anie.201708277. Epub 2017 Oct 9. 10.1002/anie.201708277 PubMed 28941028