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PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEBTet
- Backbone size w/o insert (bp) 11448
- Total vector size (bp) 13797
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNADP-Snifit
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Alt namehSPR-HaloTag7-Pro30-SNAPtag
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SpeciesSynthetic
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Insert Size (bp)2349
- Promoter CMV TetO2
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains some differences in the oriP backbone sequence compared to the depositor sequence. These differences are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBTet_NADP-Snifit-cyto was a gift from Kai Johnsson (Addgene plasmid # 113917 ; http://n2t.net/addgene:113917 ; RRID:Addgene_113917) -
For your References section:
Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 10.7554/eLife.32638 PubMed 29809136