Skip to main content
Addgene

pEBTet_NAD-Snifit-nucl
(Plasmid #113915)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113915 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEBTet
  • Backbone size w/o insert (bp) 11448
  • Total vector size (bp) 13899
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NAD-Snifit
  • Alt name
    hSPR(D41W42)-HaloTag7-Pro30-SNAPtag
  • Species
    Synthetic
  • Insert Size (bp)
    2349
  • Promoter CMV TetO2

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains some differences in the oriP backbone sequence compared to the depositor sequence. These differences are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBTet_NAD-Snifit-nucl was a gift from Kai Johnsson (Addgene plasmid # 113915 ; http://n2t.net/addgene:113915 ; RRID:Addgene_113915)
  • For your References section:

    Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 10.7554/eLife.32638 PubMed 29809136