pEBTet_NAD-Snifit-cyto
(Plasmid #113914)

• Purpose: Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells
• Depositing Lab: Kai Johnsson
• Publication: Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.

Backbone

• Vector backbone: pEBTet
• Backbone size w/o insert (bp): 11448
• Total vector size (bp): 13797
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NAD-Snifit
• Alt name: hSPR(D41W42)-HaloTag7-Pro30-SNAPtag
• Species: Synthetic
• Insert Size (bp): 2349
• Promoter: CMV TetO2

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_Fwd
• 3′ sequencing primer: BGH_Rev

Resource Information

• Supplemental Documents:
  • pEBTet-NAD-Snifit_cyto.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains some differences in the oriP backbone sequence compared to the depositor sequence. These differences are not known to affect plasmid function.