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Addgene

pAmCyan1-C1-ADCY6
(Plasmid #113905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAmCyan1-c1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8204
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adenylate Cyclase 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3504
  • Entrez Gene
    ADCY6 (a.k.a. AC6, LCCS8)
  • Promoter pCMV
  • Tag / Fusion Protein
    • Cyan (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site ECoRI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please acknowledge the Principal Investigator, cite the article in which the plasmids were described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAmCyan1-C1-ADCY6 was a gift from Anjaparavanda Naren (Addgene plasmid # 113905 ; http://n2t.net/addgene:113905 ; RRID:Addgene_113905)

  • For your References section:

    AC6 is the major adenylate cyclase forming a diarrheagenic protein complex with cystic fibrosis transmembrane conductance regulator in cholera. Thomas A, Ramananda Y, Mun K, Naren AP, Arora K. J Biol Chem. 2018 Jun 14. pii: RA118.003378. doi: 10.1074/jbc.RA118.003378. 10.1074/jbc.RA118.003378 PubMed 29903911
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