pTriEx4-ADCY6-C2 (917-1165 aa)
(Plasmid #113904)

• Purpose: His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6
• Depositing Lab: Anjaparavanda Naren
• Publication: Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003378. doi: 10.1074/jbc.RA118.003378.

Backbone

• Vector backbone: pTriEx-4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 5985
• Modifications to backbone: None
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Adenylate Cyclase 6 cytoplasmic catalytic domain 2
• Species: H. sapiens (human)
• Insert Size (bp): 747
• Mutation: Please see Depositor Comments
• Entrez Gene: ADCY6 (a.k.a. AC6, LCCS8)
• Promoter: T7, CMV
• Tag / Fusion Protein:
  • His-S (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: TriEx Dn

Resource Information

• A portion of this plasmid was derived from a plasmid made by: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge the Principal Investigator, cite the article in which the plasmids were described.

Addgene NGS found a V1002A amino acid change within the ADCY6 translation. The depositing laboratory affirms that V1002A does not affect the binding capability of the protein to CFTR that was published in their JBC paper, though they have not tested the function of this construct