pTriEx4-ADCY6-C1(306-672 aa)
(Plasmid #113903)

• Purpose: His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6
• Depositing Lab: Anjaparavanda Naren
• Publication: Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003378. doi: 10.1074/jbc.RA118.003378.

Backbone

• Vector backbone: pTriEx-4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 6339
• Modifications to backbone: None
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Adenylate Cyclase 6 cytoplasmic catalytic domain 1
• Species: H. sapiens (human)
• Insert Size (bp): 1101
• Entrez Gene: ADCY6 (a.k.a. AC6, LCCS8)
• Promoter: T7, CMV
• Tag / Fusion Protein:
  • His-S

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: TriEx Dn

Resource Information

• A portion of this plasmid was derived from a plasmid made by: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge the Principal Investigator, cite the article in which the plasmids were described.