pHR-CAG_3C-Twin-Strep
(Plasmid #113901)

• Purpose: (Empty Backbone) Mammalian protein expression
• Depositing Lab: A. Radu Aricescu
• Publication: Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 10.1038/s41596-018-0075-9.

Backbone

• Vector backbone: pHR-CAG
• Vector type: Lentiviral
• Tag / Fusion Protein:
  • 3C-Twin-Strep (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: CAG
• Tag / Fusion Protein:
  • 3C-Twin-Strep (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, AgeI (not destroyed)
• 3′ cloning site: KpnI, XhoI (not destroyed)
• 5′ sequencing primer: GTGCTGGTTATTGTGCTGTCTCATC
• 3′ sequencing primer: CATTAAAGCAGCGTATCCACATAGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This empty backbone contains a 12bp dummy insert.

Promoter/enhancer amplified from pHLsec plasmid (Addgene entry 99845); chicken B-actin promoter sequence not fully verified by Sanger sequencing.