pARF1-YFP
(Plasmid #11390)

• Depositing Lab: Joel Swanson
• Publication: Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ADP Ribosylation Factor 1
• Alt name: ARF1
• Alt name: YFP
• Species: H. sapiens (human)
• Insert Size (bp): 545
• GenBank ID: AF493881
• Entrez Gene: ARF1 (a.k.a. PVNH8)
• Tag / Fusion Protein:
  • YFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: GGTGGGAGGTCTATATAAGCAGAGCTGGTT
• 3′ sequencing primer: CCCCGGTGAACAGCTCCTCGCCCTTGCTCA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hARF1 was obtained from the UMR cDNA Resource center (www.cDNA.org).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes a YFP fusion to the C-terminus of ARF1. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP variant.

Please note that the XbaI site depicted in the map is not actually present in this plasmid.