pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP
(Plasmid #113888)

• Purpose: (Empty Backbone) Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP
• Depositing Lab: A. Radu Aricescu
• Publication: Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s41596-018-0075-9. doi: 10.1038/s41596-018-0075-9.

Backbone

• Vector backbone: pHR-CMV-TetO2
• Vector type: Lentiviral
• Selectable markers: EmGFP
• Tag / Fusion Protein:
  • 3C-Avi-His6 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: CMV-MIE-TetO2
• Tag / Fusion Protein:
  • 3C-Avi-His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, AgeI (not destroyed)
• 3′ cloning site: KpnI, XhoI (not destroyed)
• 5′ sequencing primer: CGTCGTCGTGCTCGTTTAGTG
• 3′ sequencing primer: CAAACGCACACCGGCCTTATTCCAAG

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This empty backbone contains a 12bp dummy insert.