p3E_he1a:mCherry
(Plasmid #113881)

• Purpose: p3E entry vector for Three Multisite Gateway
• Depositing Lab: Marnie Halpern
• Publication: p3E_he1a constructs (unpublished)

Backbone

• Vector backbone: pDONR P2R-P3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2640
• Total vector size (bp): 4389
• Vector type: Zebrafish Danio expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 10 ug/ml kanamycin final concentration of LB media
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: he1a
• Alt name: hatching gland promoter mCherry
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1749
• Entrez Gene: he1.1 (a.k.a. cb284, he1, he1a, wu:cegs2099, zgc:111847)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • p3E_he1a-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:mRFP-NTRmut;HE-mCherry

Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner​."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93