p3E_he1a:ECFP
(Plasmid
#113880)
-
Purposep3E entry vector for Three Multisite Gateway
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 113880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDONR P2R-P3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2640
- Total vector size (bp) 4439
-
Vector typeZebrafish Danio expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructions10 ug/ml kanamycin final concentration of LB media
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehe1a
-
Alt namehatching gland promoter mCerulean
-
SpeciesD. rerio (zebrafish)
-
Insert Size (bp)1799
-
Entrez Genehe1.1 (a.k.a. cb284, he1, he1a, wu:cegs2099, zgc:111847)
-
Tag
/ Fusion Protein
- ECFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned from the construct provided by Jeff Mumm's Lab, Tol2 5xUAS-mCFP-p2A-NTRmut;HE:mCerulean
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p3E_he1a:ECFP was a gift from Marnie Halpern (Addgene plasmid # 113880 ; http://n2t.net/addgene:113880 ; RRID:Addgene_113880)