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pARF6(Q67L)-CFP
(Plasmid #11387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADP Ribosylation Factor 6
  • Alt name
    ARF6(Q67L)
  • Alt name
    CFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    527
  • GenBank ID
    NP_001654
  • Entrez Gene
    ARF6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GGTGGGAGGTCTATATAAGCAGAGCTGGTT
  • 3′ sequencing primer CCCCGGTGAACAGCTCCTCGCCCTTGCTCA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hARF6 was obtained from the UMR cDNA Resource center (www.cDNA.org) and mutagenized using site-directed mutagenesis.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes a CFP fusion to the C-terminus of constitutively-active ARF6. CFP is the monomeric (A207K) version of ECFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pARF6(Q67L)-CFP was a gift from Joel Swanson (Addgene plasmid # 11387 ; http://n2t.net/addgene:11387 ; RRID:Addgene_11387)

  • For your References section:

    A Phosphatidylinositol-3-Kinase-Dependent Signal Transition Regulates ARF1 and ARF6 during Fcgamma Receptor-Mediated Phagocytosis. Beemiller P, Hoppe AD, Swanson JA. PLoS Biol. 2006 May 9. 4(6):e162. 10.1371/journal.pbio.0040162 PubMed 16669702
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