pMuLE_ENTR_CBF-GLuc_L3-L2
(Plasmid #113868)

• Purpose: Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: pMuLE ENTR MCS L3-L2
• Backbone size w/o insert (bp): 2665
• Total vector size (bp): 3644
• Vector type: Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CBF (notch dependent promoter)
• Alt name: CBFRE
• Insert Size (bp): 416

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13

Gene/Insert 2

• Gene/Insert name: GLuc
• Alt name: Gaussia Luciferase
• Insert Size (bp): 596

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • pMuLE_ENTR_CBF-GLuc_L3-L2.gb
• A portion of this plasmid was derived from a plasmid made by: pCMV-Gluc (N8081S), was acquired from New England Biolabs. CBF:H2B-Venus was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 44211). Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry