pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
(Plasmid #113864)

• Purpose: Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: pMuLE ENTR MCS R4-R3
• Backbone size w/o insert (bp): 2775
• Total vector size (bp): 4959
• Vector type: Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PTCH1 (hedgehog dependent promoter)
• Alt name: hPtch1 prom wt
• Species: H. sapiens (human)
• Insert Size (bp): 1375

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13

Gene/Insert 2

• Gene/Insert name: mTurquoise2
• Insert Size (bp): 817

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3.gb
  • Plasmid History
• A portion of this plasmid was derived from a plasmid made by: pGL3b hPtch1 prom wt was a gift from Fritz Aberger. pmTurquoise2-NES was a gift from Dorus Gadella (Addgene plasmid#36206). Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Winklmayr M, Schmid C, Laner-Plamberger S, Kaser A, Aberger F, Eichberger T, et al. Non-consensus GLI binding sites in Hedgehog target gene regulation. BMC molecular biology. 2010;11(1):2