Crimson/P2A-Control (pSLIK3)
(Plasmid
#113858)
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PurposeAn isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSLIK-Zeo
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Backbone manufacturerIain Fraser (Addgene plasmid # 25736)
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Vector typeLentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE2-Crimson
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- P2A/Crimson (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/02/11/259739 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Crimson/P2A-Control (pSLIK3) was a gift from Richard S Myers (Addgene plasmid # 113858 ; http://n2t.net/addgene:113858 ; RRID:Addgene_113858) -
For your References section:
Herpes ICP8 protein stimulates homologous recombination in human cells. Valledor M, Myers RS, Schiller PC. PLoS One. 2018 Aug 15;13(8):e0200955. doi: 10.1371/journal.pone.0200955. eCollection 2018. 10.1371/journal.pone.0200955 PubMed 30110337