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Addgene

Crimson/P2A-ICP8 (pSLIK1)
(Plasmid #113856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK-Zeo
  • Backbone manufacturer
    Iain Fraser (Addgene plasmid # 25736)
  • Modifications to backbone
    The Synaptase gene is fused to E2-Crimson fluorescent protein gene through a P2A linker to follow synaptase expression without tethering E2-Crimson to the synaptase protein.
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ICP8
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • P2A/Crimson (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/02/11/259739 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Crimson/P2A-ICP8 (pSLIK1) was a gift from Richard S Myers (Addgene plasmid # 113856 ; http://n2t.net/addgene:113856 ; RRID:Addgene_113856)

  • For your References section:

    Herpes ICP8 protein stimulates homologous recombination in human cells. Valledor M, Myers RS, Schiller PC. PLoS One. 2018 Aug 15;13(8):e0200955. doi: 10.1371/journal.pone.0200955. eCollection 2018. 10.1371/journal.pone.0200955 PubMed 30110337
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