pREST-donor
(Plasmid
#113807)
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PurposeCRISPR donor plasmid to tag human transcription factor REST with GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR Blunt TOPO
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Backbone manufacturerInvitrogen
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameREST homology arms flanking EGFP-IRES-Neo cassette
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SpeciesH. sapiens (human)
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Mutationrs4864608
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Entrez GeneREST (a.k.a. DFNA27, GINGF5, HGF5, NRSF, WT6, XBR)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13pUC-rev
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning source: CHM1htert.
Use with plasmid pREST.1.0-gDNA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREST-donor was a gift from Kevin White (Addgene plasmid # 113807 ; http://n2t.net/addgene:113807 ; RRID:Addgene_113807)
