pET28a-IMX-DogTag
(Plasmid
#113765)
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PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5366
- Total vector size (bp) 5642
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIMX-DogTag
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SpeciesSynthetic
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Insert Size (bp)276
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-IMX-DogTag was a gift from Mark Howarth (Addgene plasmid # 113765 ; http://n2t.net/addgene:113765 ; RRID:Addgene_113765) -
For your References section:
SnoopLigase peptide-peptide conjugation enables modular vaccine assembly. Andersson AC, Buldun CM, Pattinson DJ, Draper SJ, Howarth M. Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.1038/s41598-019-40985-w. 10.1038/s41598-019-40985-w PubMed 30874593
