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Addgene

FLAG-BCL9
(Plasmid #113759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BCL9
  • Species
    H. sapiens (human)
  • Entrez Gene
    BCL9 (a.k.a. LGS)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-BCL9 was a gift from Mariann Bienz (Addgene plasmid # 113759 ; http://n2t.net/addgene:113759 ; RRID:Addgene_113759)

  • For your References section:

    The function of BCL9 in Wnt/beta-catenin signaling and colorectal cancer cells. de la Roche M, Worm J, Bienz M. BMC Cancer. 2008 Jul 15;8:199. doi: 10.1186/1471-2407-8-199. 10.1186/1471-2407-8-199 PubMed 18627596
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