pGEM-gate
(Plasmid
#113758)
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PurposeGateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113758 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3048
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Vector typeGateway Destination Vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsMay use DB3.1 cells instead of ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegateway cassette
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Insert Size (bp)1703
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-gate was a gift from Magdalena Bezanilla (Addgene plasmid # 113758 ; http://n2t.net/addgene:113758 ; RRID:Addgene_113758) -
For your References section:
Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Mallett DR, Chang M, Cheng X, Bezanilla M. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. 10.1002/pld3.168 PubMed 31523744