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Addgene

pBABE puro mouse IRS-1 myc Notes

Addgene's diagnostic digest

This image depicts the NEB 1kb ladder and a double digest of the plasmid
pBABE puro mouse IRS-1 myc with BamHI-HF and SalI-HF. Please note that the plasmid map from the depositing scientist indicates that a 3-way ligation was performed to construct this plasmid because there is an endogenous BamHI site in the IRS-1 sequence. We see the expected digest pattern for this plasmid.