pBABE puro mouse IRS-2 myc
(Plasmid #11373)

• Depositing Lab: Ronald Kahn
• Publication: Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Backbone

• Vector backbone: pBABE puro
• Backbone manufacturer: pBABE-puro is available at Addgene
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRS-2
• Alt name: Insulin Receptor Substrate 2
• Alt name: IRS2
• Species: M. musculus (mouse)
• Insert Size (bp): 4244
• Entrez Gene: Irs2 (a.k.a. Irs-2)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Morris White provided the IRS-2 gene in the form of the plasmid pCMV IRS-2.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

(Kahn lab plasmid name = pBabe puro IRS2 Myc)

Addgene NGS results found a G384E variant and an S172 insertion compared to the NCBI reference [NP_001074681.1].