EF.hICN1
(Plasmid #113715)

• Purpose: Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: EF.hICN1.CMV.GFP
• Total vector size (bp): 9166
• Modifications to backbone: Removed CMV.GFP
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • EF.hICN1.gb
  • Plasmid History
• A portion of this plasmid was derived from a plasmid made by: EF.hICN1.CMV.GFP was a gift from Linzhao Cheng (Addgene plasmid #17623).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The hICN1 insert contains a difference that results in A184S compared to the NCBI reference. This difference does not impact the function of the plasmid.