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Addgene

pBABE puro mouse IRS-2
(Plasmid #11371)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    pBABE-puro is available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRS-2
  • Alt name
    Insulin Receptor Substrate 2
  • Alt name
    IRS2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4244
  • Entrez Gene
    Irs2 (a.k.a. Irs-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Morris White provided the IRS-2 gene in the form of the plasmid pCMV IRS-2.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

(Kahn lab plasmid name = pBabe puro mIRS2)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro mouse IRS-2 was a gift from Ronald Kahn (Addgene plasmid # 11371 ; http://n2t.net/addgene:11371 ; RRID:Addgene_11371)

  • For your References section:

    Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR. Mol Cell Biol. 2001 Jan . 21(1):26-38. 10.1128/MCB.21.1.26-38.2001 PubMed 11113178
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