pMuLE_ENTR_CBF-tdTomato_L3-L2
(Plasmid #113708)

• Purpose: Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: pMuLE ENTR SV40 tdTomato L3-L2
• Backbone size w/o insert (bp): 4403
• Total vector size (bp): 4515
• Vector type: Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBF (notch dependent promoter)
• Alt name: CBFRE
• Insert Size (bp): 446

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CBF:H2B-Venus was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 44211). Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry