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pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA
(Plasmid #113692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Agilent Technologies
  • Total vector size (bp) 7524
  • Vector type
    Mouse Targeting, AAV, Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SaCas9
  • Entrez Gene
    NEWENTRY
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    SaCas9 gRNA Cassette
  • Entrez Gene
    NEWENTRY

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA from Feng Zhang plasmid #61591, Cre-Lox sites from Karl Deisseroth, pAAV-Ef1a-DIO EYFP plasmid #27056.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CREB gRNA sequence: GGAGCAGACAACCAGCAGAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA was a gift from Jonathan Ploski (Addgene plasmid # 113692 ; http://n2t.net/addgene:113692 ; RRID:Addgene_113692)

  • For your References section:

    The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Kumar N, Stanford W, de Solis C, Aradhana, Abraham ND, Dao TJ, Thaseen S, Sairavi A, Gonzalez CU, Ploski JE. Front Mol Neurosci. 2018 Nov 13;11:413. doi: 10.3389/fnmol.2018.00413. eCollection 2018. 10.3389/fnmol.2018.00413 PubMed 30483052
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