pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
(Plasmid #113691)

• Purpose: U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
• Depositing Lab: Jonathan Ploski
• Publication: Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413. doi: 10.3389/fnmol.2018.00413. eCollection 2018.

Backbone

• Vector backbone: AAV
• Backbone manufacturer: Agilent Technologies
• Total vector size (bp): 7522
• Vector type: AAV, Cre/Lox, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SaCas9
• Alt name: SauCas9
• Species: Staphylococcus aureus
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA.gb
• A portion of this plasmid was derived from a plasmid made by: pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA from Feng Zhang plasmid #61591, Cre-Lox sites from Karl Deisseroth, pAAV-Ef1a-DIO EYFP plasmid #27056,
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry