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Addgene

pcDNA-ncpGCaMP6s
(Plasmid #113674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ncpGCaMP6s
  • Species
    Synthetic
  • Insert Size (bp)
    1245
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-ncpGCaMP6s was a gift from Robert Campbell (Addgene plasmid # 113674 ; http://n2t.net/addgene:113674 ; RRID:Addgene_113674)

  • For your References section:

    A bioluminescent Ca2+ indicator based on a topological variant of GCaMP6s. Qian Y, Rancic V, Wu J, Ballanyi K, Campbell RE. Chembiochem. 2018 Jun 22. doi: 10.1002/cbic.201800255. 10.1002/cbic.201800255 PubMed 29934970
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