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Addgene

pLX_TRC311
(Plasmid #113668)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113668 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLX_TRC311
  • Backbone manufacturer
    Genetic Perturbation Platform
  • Backbone size (bp) 9879
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter EF1a
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    depositor recommends Carbenicillin (100ug/ml) and chloramphenicol (50ug/ml) for bacterial resistance.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX_TRC311 was a gift from John Doench (Addgene plasmid # 113668 ; http://n2t.net/addgene:113668 ; RRID:Addgene_113668)