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Addgene

anti-FX(a) Emi HC
(Plasmid #113665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1(+) Hygro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Emicizumab anti-FX/FXa heavy chain
  • Species
    humanized monoclonal antibody
  • Promoter CMV Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-FX(a) Emi HC was a gift from Robert Peters (Addgene plasmid # 113665 ; http://n2t.net/addgene:113665 ; RRID:Addgene_113665)

  • For your References section:

    Intrinsic differences between FVIIIa mimetic bispecific antibodies and FVIII prevent assignment of FVIII-equivalence. Leksa NC, Aleman MM, Goodman A, Rabinovich D, Peters R, Salas J. J Thromb Haemost. 2019 Mar 18. doi: 10.1111/jth.14430. 10.1111/jth.14430 PubMed 30887655
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