pDA12
(Plasmid
#113663)
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Purpose(Empty Backbone) Cloning vector for bacterial expression with Pdhfr promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAP2
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Vector typeBacterial Expression
- Promoter Pdhfr
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBRrevBam
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDA12 was a gift from Miguel Valvano (Addgene plasmid # 113663 ; http://n2t.net/addgene:113663 ; RRID:Addgene_113663) -
For your References section:
A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. Aubert DF, Flannagan RS, Valvano MA. Infect Immun. 2008 May;76(5):1979-91. doi: 10.1128/IAI.01338-07. Epub 2008 Mar 3. 10.1128/IAI.01338-07 PubMed 18316384
