pBABE-hygro NM23 H1 Pro
(Plasmid #11366)

• Depositing Lab: Ronald Kahn
• Publication: Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 96(26):14911-8.

Backbone

• Vector backbone: pBABE-hygro
• Backbone size w/o insert (bp): 5558
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NM23 H1
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: Pro96 mutation to Ser (reduces GAP activity).
• Entrez Gene: NME1 (a.k.a. AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry