pET-51b(+)_NAD-Snifit
(Plasmid
#113658)
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PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET51-b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5218
- Total vector size (bp) 7537
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNAD-Snifit
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Alt namehSPR(D41W42)-HaloTag7-Pro30-SNAPtag
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SpeciesSynthetic
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Insert Size (bp)2475
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer pET-RP (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-51b(+)_NAD-Snifit was a gift from Kai Johnsson (Addgene plasmid # 113658 ; http://n2t.net/addgene:113658 ; RRID:Addgene_113658) -
For your References section:
Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 10.7554/eLife.32638 PubMed 29809136
