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Addgene

pDCAF-BC
(Plasmid #113651)

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Item Catalog # Description Quantity Price (USD)
Plasmid 113651 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pSB1C3
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ΔguaB E. coli
  • Growth instructions
    Growing in M9CG media with the appropriate Methylxanthines is recommended in order to maintain the plasmid. Can be grown on LB but must be grown in strain ΔguaB (Addgene #113653).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ndmB and ndmC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDCAF-BC was a gift from Jeffrey Barrick (Addgene plasmid # 113651 ; http://n2t.net/addgene:113651 ; RRID:Addgene_113651)

  • For your References section:

    Bioassay for Determining the Concentrations of Caffeine and Individual Methylxanthines in Complex Samples. Gutierrez AE, Shah P, Rex AE, Nguyen TC, Kenkare SP, Barrick JE, Mishler DM. Appl Environ Microbiol. 2019 Nov 14;85(23). pii: AEM.01965-19. doi: 10.1128/AEM.01965-19. Print 2019 Dec 1. 10.1128/AEM.01965-19 PubMed 31540989
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