pRF0G-Tyr
(Plasmid
#113644)
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PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepACYC184. p15A
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMjYRS and tRNA gene
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRF0G-Tyr was a gift from Jeffrey Barrick (Addgene plasmid # 113644 ; http://n2t.net/addgene:113644 ; RRID:Addgene_113644) -
For your References section:
Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE. ACS Synth Biol. 2016 Sep 20. 10.1021/acssynbio.6b00192 PubMed 27648665