pRXG
(Plasmid
#113643)
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PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE-80L. ColE1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDO NOT USE CAM/CAT; This resistance gene is not functional
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1-linker-sfGFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRXG was a gift from Jeffrey Barrick (Addgene plasmid # 113643 ; http://n2t.net/addgene:113643 ; RRID:Addgene_113643) -
For your References section:
Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE. ACS Synth Biol. 2016 Sep 20. 10.1021/acssynbio.6b00192 PubMed 27648665
