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Addgene

pRYG
(Plasmid #113642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-80L. ColE1
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DO NOT USE CAM/CAT; This resistance gene is not functional
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP1-linker-sfGFP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRYG was a gift from Jeffrey Barrick (Addgene plasmid # 113642 ; http://n2t.net/addgene:113642 ; RRID:Addgene_113642)

  • For your References section:

    Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE. ACS Synth Biol. 2016 Sep 20. 10.1021/acssynbio.6b00192 PubMed 27648665
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