pBABE-hygro NM23 H1 WT
(Plasmid
#11364)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-hygro
- Backbone size w/o insert (bp) 5558
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNM23 H1
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SpeciesH. sapiens (human)
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Insert Size (bp)500
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Entrez GeneNME1 (a.k.a. AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-hygro NM23 H1 WT was a gift from Ronald Kahn (Addgene plasmid # 11364 ; http://n2t.net/addgene:11364 ; RRID:Addgene_11364) -
For your References section:
Interaction of the Ras-related protein associated with diabetes rad and the putative tumor metastasis suppressor NM23 provides a novel mechanism of GTPase regulation. Zhu J, Tseng YH, Kantor JD, Rhodes CJ, Zetter BR, Moyers JS, Kahn CR. Proc Natl Acad Sci U S A. 1999 Dec 21. 96(26):14911-8. 10.1073/pnas.96.26.14911 PubMed 10611312