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Addgene

pTargetF-FRT
(Plasmid #113637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTargetF
  • Total vector size (bp) 2116
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sgRNA-FRT
  • gRNA/shRNA sequence
    CTATACTTTCTAGAGAAT - flippase recognition target (FRT)
  • Species
    Synthetic
  • Promoter pJ23119

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer pTarget-1
  • 3′ sequencing primer pTarget-2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTargetF-FRT was a gift from Olivier Lichtarge (Addgene plasmid # 113637 ; http://n2t.net/addgene:113637 ; RRID:Addgene_113637)

  • For your References section:

    CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing. Swings T, Marciano DC, Atri B, Bosserman RE, Wang C, Leysen M, Bonte C, Schalck T, Furey I, Van den Bergh B, Verstraeten N, Christie PJ, Herman C, Lichtarge O, Michiels J. Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5. 10.1038/s41467-018-04651-5 [pii] PubMed 29884781
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