pDAI-SceI-SacB
(Plasmid #113635)

• Purpose: Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB
• Depositing Lab: Miguel Valvano
• Publication: Aubert et al Methods Mol Biol. 2014;1197:311-27. doi: 10.1007/978-1-4939-1261-2_18.

Backbone

• Vector backbone: pDAI-SceI
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sacB
• Mutation: Please see Depositor Comments
• Promoter: sacB promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: GCCTTCT TGACGAGTTCTTCTG
• 3′ sequencing primer: TCGAGCAAGCTGCAGTTATTTGTTAAC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function