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Purpose(Empty Backbone) An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMLBAD
- Backbone size (bp) 6775
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Vector typeBacterial Expression
- Promoter PrhaB
Growth in Bacteria
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Bacterial Resistance(s)Trimethoprim
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer None
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSCrhaB2 was a gift from Miguel Valvano (Addgene plasmid # 113634 ; http://n2t.net/addgene:113634 ; RRID:Addgene_113634) -
For your References section:
An expression vector containing a rhamnose-inducible promoter provides tightly regulated gene expression in Burkholderia cenocepacia. Cardona ST, Valvano MA. Plasmid. 2005 Nov;54(3):219-28. doi: 10.1016/j.plasmid.2005.03.004. Epub 2005 May 31. 10.1016/j.plasmid.2005.03.004 PubMed 15925406
