Skip to main content
Addgene

pBABE puro mouse IRS-4
(Plasmid #11363)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    pBABE-puro is available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRS-4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3700
  • GenBank ID
    NM_010572
  • Entrez Gene
    Irs4 (a.k.a. IRS-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse IRS-4 genomic DNA was screened from the BAC genomic DNA library (Genome Systems Inc. St. Louis, Mo.) by PCR with specific primers for mouse IRS-4 genomic DNA. The AvrII-ClaI fragment encompassing the entire exon 1 of mouse IRS-4 gene was blunt ended and ligated into the SnaBI site in pBABE-puro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro mouse IRS-4 was a gift from Ronald Kahn (Addgene plasmid # 11363 ; http://n2t.net/addgene:11363 ; RRID:Addgene_11363)

  • For your References section:

    Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR. Mol Cell Biol. 2001 Jan . 21(1):26-38. 10.1128/MCB.21.1.26-38.2001 PubMed 11113178
Related items:
Commonly requested with: