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Addgene

PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
(Plasmid #113612)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113612 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S1P1

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 113612 ; http://n2t.net/addgene:113612 ; RRID:Addgene_113612)
  • For your References section:

    A rapid fluorogenic GPCR-beta-arrestin interaction assay. Zhang Q, Zheng YW, Coughlin SR, Shu X. Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 2018 Feb 23. 10.1002/pro.3385 PubMed 29411438