pBS rat IRS-3
(Plasmid
#11361)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRS-3
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Alt nameInsulin Receptor Substrate 3
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Alt nameIRS3
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1484
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GenBank IDNM_032074
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Entrez GeneIrs3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
(Kahn lab plasmid name = pBS IRS3)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS rat IRS-3 was a gift from Ronald Kahn (Addgene plasmid # 11361 ; http://n2t.net/addgene:11361 ; RRID:Addgene_11361) -
For your References section:
Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR. Mol Cell Biol. 2001 Jan . 21(1):26-38. 10.1128/MCB.21.1.26-38.2001 PubMed 11113178
Map uploaded by the depositor.
