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PurposePPI reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113608 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePAR1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 113608 ; http://n2t.net/addgene:113608 ; RRID:Addgene_113608) -
For your References section:
A rapid fluorogenic GPCR-beta-arrestin interaction assay. Zhang Q, Zheng YW, Coughlin SR, Shu X. Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 2018 Feb 23. 10.1002/pro.3385 PubMed 29411438