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pBS human IRS-1
(Plasmid #11359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11359 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRS-1
  • Alt name
    Insulin Receptor Substrate 1
  • Alt name
    IRS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5800
  • GenBank ID
    NM_005544
  • Entrez Gene
    IRS1 (a.k.a. HIRS-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains full-length IRS-1 protein. Cloned from human muscle cDNA library. (Kahn lab plasmid name = phIRS1-1)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS human IRS-1 was a gift from Ronald Kahn (Addgene plasmid # 11359 ; http://n2t.net/addgene:11359 ; RRID:Addgene_11359)

  • For your References section:

    Human skeletal muscle insulin receptor substrate-1. Characterization of the cDNA, gene, and chromosomal localization. Araki E, Sun XJ, Haag BL, Chuang LM, Zhang Y, Yang-Feng TL, White MF, Kahn CR. Diabetes. 1993 Jul . 42(7):1041-54. 10.2337/diabetes.42.7.1041 PubMed 8513971
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