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Addgene

BK beta4
(Plasmid #113568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6V5HisA
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcnmb4
  • Alt name
    beta4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    632
  • Entrez Gene
    Kcnmb4 (a.k.a. 1700058G18Rik, 2900045G12Rik)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BK beta4 was a gift from Robert Brenner (Addgene plasmid # 113568 ; http://n2t.net/addgene:113568 ; RRID:Addgene_113568)

  • For your References section:

    Mechanism of beta4 subunit modulation of BK channels. Wang B, Rothberg BS, Brenner R. J Gen Physiol. 2006 Apr . 127(4):449-65. 10.1085/jgp.200509436 PubMed 16567466
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