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Addgene

BK beta1
(Plasmid #113565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcnmb1
  • Alt name
    beta1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    597
  • Entrez Gene
    Kcnmb1 (a.k.a. BKbeta1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BK beta1 was a gift from Robert Brenner (Addgene plasmid # 113565 ; http://n2t.net/addgene:113565 ; RRID:Addgene_113565)

  • For your References section:

    An S6 mutation in BK channels reveals beta1 subunit effects on intrinsic and voltage-dependent gating. Wang B, Brenner R. J Gen Physiol. 2006 Dec;128(6):731-44. doi: 10.1085/jgp.200609596. 10.1085/jgp.200609596 PubMed 17130522
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